Mechanisms of paradoxical sleep
(d) Deprivation of P.S. in the pontile animal
Since P.S. is the only form of sleep in pontile animals we turned to
a study of the effects of deprivation. Our technique was as follows: at
the beginning of each phase of P.S. the fall in nuchal EMG activity, by
the intermediary of an Oneirograph, switches on a stimulator which applies
electric shocks to the animal's log. The strength of these shocks is regulated
to arouse the animal, in which case the reappearance of EMG activity automatically
cuts out the stimulation.
Results: Deprivation was maintained for a maximum of 8-9 h. It
appeared that when P.S. is suppressed by a shock it tends to recur at
ever shorter intervals. At first these intervals are similar to those
between periods of P.S. in controls. but after some hours they become
so short that 40 60 sec after being wakened by the shock the animal falls
into a new phase of P.S. (Fig. 21). Thus the
total number of incipient phases of P.S. was as much as 155 in sessions
that lasted for 9 h, and P.S. can hardly be suppressed for longer periods
than this in view of the very great number of shocks required. When the
stimuli were stopped, P.S. returned immediately. Its average duration
was then slightly more than that of controls (7 as against 6 min), and
its periodicity remained at a higher level for 2-3 h and then returned
to normal.
A result such as this exemplifies the necessary nature of P.S. in pontile
animals, it suggests an active process situated in the brain stem, the
efficacy of which increases as its effect is suppressed.
Next page
|