Welcome to WAKING Team of the Lyon Neurosciences Research Center

Integrated Physiology of the Brain Arousal Systems

Overview

Overview

Our goal is to unravel the mechanisms of wake and associated pathologies: narcolepsy, excessive daytime sleepiness, insomnia, metabolic disorders, sudden infant death syndrome.

From a basic research standpoint, we dissect the mechanisms of wake through the study of the specific, complementary and synergistic roles of histaminergic and orexinergic neurons in particular. Using specific knock out mice and detailed behavioral and electroencephalographic measures, we will determine how the two systems interact synergistically and integrate into the central mechanisms of sleep-wake alteration (JS Lin, K Sakai). We also use intracellular recordings, local field potentials and biosensors to study the properties of the cortical network during different types of behavioral states, the respective role of diffuse ascending systems and specific cerebral nuclei, and the mechanisms and effects of extra-synaptic variations of neurotransmitter.

From a physio-pathological and translational research standpoint, we work on 1) developing therapeutic approaches for narcolepsy and excessive daytime sleepiness using our knowledge of histaminergic and orexinergic systems (P Franco, JS Lin), 2) investigating the interactions between sleep loss and metabolic disorders in lean subjects and obese patients and in animal models combining sleep/wake and metabolic alterations (K. Spiegel), and 3) identifying new signaling pathways and biomarkers associated with wake thanks to Drosophila molecular genetics (L. Seugnet).

Our work is thus characterized by the use multidisciplinary methodological approaches: electrophysiology and behavior, biosensors, clinical investigations, molecular genetics, and the development of tools for both diagnostic and therapy.

Methodology

  • Electrophysiology (EEG, local field potential, intra and extracellular recordings in behavioral mice and cats)
  • Polysomnography in animals (mouse, cat) and humans (adult,child)
  • Biosensors (electrochemistry)
  • Immunohistochemistry
  • Neuro-behavioural testing
  • Neuropharmacology
  • Cognitive, metabolic and endocrine testing in humans
  • Animal models for sleep disorders (mouse, cat)
  • Sleep molecular genetics in Drosophila

Keywords : waking, arousal, sleep, slow wave sleep, REM sleep, cortical activation, posterior hypothalamus, histamine, orexin, sleep-wake disorder, somnolence, waking substance, mesopontine tegmentum, neurophysiology, cat, mouse

Brief history of the laboratory

Our laboratory was initially funded and directed by Pr. Michel Jouvet and focused on the neurobiological mechanisms regulating sleep and paradoxical sleep in particular, (INSERM-U52/CNRS-URA1195, Département de Médecine Expérimentale à l’Université Claude Bernard, 1957-1998, then INSERM-U480, 1998-2002, directed by Dr. Raymond Cespuglio). In 2004 we created a new team: “integrated physiology of brain arousal system” focused on the central mechanisms responsible for the physiology and the maintenance of wake (INSERM-U628 directed by Jian-Sheng Lin). Since January 2011, our team is part of the Lyon Neuroscience Research Center (Team WAKING). Our research is multi-disciplinary and includes fundamental as well as clinical aspects: mouse of models of somnolence and narcolepsy using integrated physiology and state of the art electrophysiology, development of biosensors, pediatric narcolepsy, metabolic consequences of extended wake, molecular-genetics of sleep/wake regulation.

Scientific presentation of the laboratory

Team

Jian-Sheng LIN

Jian-Sheng LIN

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Laurent SEUGNET

Laurent SEUGNET

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People

5

INSERM researchers

6

University clinician-researchers

10

technical staff

6

Post-docs and PhD students

Contact waking team

CRNL
Equipe WAKING
95, Boulevard Pinel
Bâtiment 462 - Neurocampus
8 avenue Rockefeller
69500 Bron
Auvergne-Rhône-Alpes, FRANCE

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