Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons
Luppi P.H., Fort P., Jouvet M.
Brain Res. 534 (1-2) pages : 209-224 (1990)
TABLE OF CONTENTS

Introduction

Materials and Methods

Materials and Methods

Results

(A) Injection sites

(B) Retrograde labeling

(C) Artefactual labeling due to uptake by fibers of passage

(D) Anterograde tracing

(E) Double immunostaining technique

Discussion

Figures

PRINT
Printable version

Figure 5A

A-D: color photomicrographs illustrating CTb-labeled cells charaeterized hy black punctate labeling and AADC-(A-C) and ChAT (D)-immunoreactive cells characterized by diffuse light brown staining. Double-labeled cells are easily distinguished in the nucleus raphe magnus (A-C) and the pedunculopontine nucleus (D) in the pons. The arrow in A indicates the double-labeled cell shown at a higher magnification in B.

Bars = 50 µm (A) and 25 µm (B-D).

Nomenclature according to Berman 4.

Next page