Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons
Luppi P.H., Fort P., Jouvet M.
Brain Res. 534 (1-2) pages : 209-224 (1990)


Materials and Methods

Materials and Methods


(A) Injection sites

(B) Retrograde labeling

(C) Artefactual labeling due to uptake by fibers of passage

(D) Anterograde tracing

(E) Double immunostaining technique



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Figure 4

Click on photomicrographs A,B,C or D to get it bigger

A-D: photomicrographs of 50 µm thick vibratome sections in a cat with a 0.2 µl pressure injection of CTb in the rostral part of the inferior olivary complex and 48 h of survival including 24 h of colchicine treatment (case F111). Note the very extensive labeling of the dendritic trees and the well preserved morphology of the retrogradely labeled cells found in the interstitial nucleus of Cajal (A), the posterior pretectal nucleus (B and C) and the medial preoptic area (D).

Bars = 200 µm (A) and 50 µm (B,C,D).

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