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Materials and methods
Results were obtained from 52 chronic cats ( 12 normal cats, 11 totally
or partially decorticate, 20 with total or partial brain stem section,
7 with partial coagulation of the brain stem, and 2 which had undergone
total ablation of the cerebellum). These animals were implanted with nickel-chrome
multipolar sub cortical electrodes and steel cortical electrodes, and
were all studied for more than one week. Decortication was carried out
in one operation by suction after removal of the skull. An acrylic resin
plastic roof was then inserted in place of the bone and the subcortical
electrodes were thus kept at that level (Jouvet, Michel and Courjon, 1959a).
The brain stem was sectioned by means of a stereotaxically oriented cutting
blade, and coagulation was obtained by means of anodal electrolytic lesion.
In some cases, the entire part of the encephalon situated in front of
the midbrain was removed by aspiration. Poikilotherm, mesencephalic or
pontile animals were kept warm and always studied at normal temperatures
between 37° and 39°. The cats were placed in soundproof cages
and EEG recording was carried out daily for 6 to 12 hours. As a routine
measure, together with the EEG, the EMG was recorded by means of a bipolar
electrode permanently mounted at the level of the neck muscles. The eye
movements were also recorded by electrodes fixed on both sides of the
eye balls. The behaviour of the animals (pupils, nictitating membranes,
posture, etc.) could also be observed through glass. In all cases, the
siting of the electrodes and the topography of the lesions were checked
histologically.
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